Fig. 2: nCas9 induces indel mutations at the target sites.
From: Precise base editing without unintended indels in human cells and mouse primary myoblasts
a Wild-type Cas9 and nCas9 generate indels at the seven target sites in human cells. b, c, d, e Alignments of the top three indel mutant sequences induced by Cas9 and nCas9 in representative EIF3D, MYOCD, HEK3, and HBB-E2 targets. Red arrowheads and dotted lines, cleavage sites induced by SpCas9; red letter, insertion or substitution sequence; blue letter, PAM sequence; underline, target sequences. Control indicates the untreated group. Each dot represents an individual target experiment. The data are shown as the mean ± S.D. of three independent experiments.
