Fig. 4: CMP-conjugated dCas9-based base editors promote nucleotide substitutions without unintended indels.
From: Precise base editing without unintended indels in human cells and mouse primary myoblasts
a–f C-to-T substitutions and indel efficiencies of CBE and BP variants at Site18, HBB, and RNF2. g–i A-to-G conversions and indel frequencies of ABE and AP variants at Site19, HBB-E2, and HEK2. The data are shown as the mean ± S.D. of four independent experiments. Each dot represents an individual target experiment. m, n Base editing and indel frequencies of CMP-conjugated dCas9-based CBE variants at 23 target sites in HEK293T cells. o Fold changes in (C–T) conversions induced by all CMP-conjugated dCas9-based CBE variants at each target. p, q Comparisons of base editing and indel frequencies induced by CMP-conjugated dCas9-based ABE variants at 21 target sites in HEK293T cells. r Fold changes in (A–G) conversions of CMP-conjugated dCas9-based ABE variants at each target. Each dot represents an individual target experiment, and (m–r) include the means of the target values in (a–l). Control indicates the untreated group. The data are shown as the mean ± S.E.M. of the independent experiments in (o–r).
