Table 1 Genes involved in hematopoiesis identified using forward genetic screening.
From: Exploring hematopoiesis in zebrafish using forward genetic screening
Hematopoietic process | Mutant name | Gene | Phenotype | Readout for screening | Biological character of the mutants | Refs. |
|---|---|---|---|---|---|---|
Primitive erythropoiesis | moonshine | tif1r | Bloodless | gata1 WISH | Apoptosis in erythroid progenitor cells | |
vlad tepes | gata1 | Bloodless | Blood cell count | Inability to bind to the promoter of its target gene | ||
t21384 | tal1 | Vascular defects | Endothelial alkaline phosphatase activity | Involved in the differentiation of red blood cells | ||
Primitive myelopoiesis | mummy | dhx8 | No blood circulation | cbfb and l-plastin WISH | Affects mRNA splicing and cell division during mitosis | |
moli | cebp-a | Decreased macrophage and neutrophils | Neutral red, Sudan black B staining | Cell cycle arrest of myeloid progenitors | ||
Early endothelial and blood cells | cloche | PAS-domain-containing bHLH transcription factor | Blood reduction and vascular defects | GATA-2 expression | Defects in the development of the heart, vasculature and blood | |
hi2335 | plcg1 | Bloodless | cmyb WISH | Lack of artery specification | ||
hi3714 | tbx16 | Bloodless | cmyb WISH | Lack of axial vascular organization | ||
hi1618 | hdac1 | Bloodless | cmyb WISH | Defect in vessel or artery formation | ||
HSC specification | sd45 | supt16h | Lack of HSCs | runx1 WISH | Enlarged hindbrain and curved tail | |
HSC maintenance | rumba | C2H2 zinc finger protein | Lack of T lymphocytes | rag1 WISH | Delayed cell cycle | |
samba | has3 | Lack of T lymphocytes | rag1 WISH | Cell arrested in M phase | ||
lack | sae1 | Lack of T lymphocytes | rag1 WISH | Impaired SUMOylation | ||
116 | rpc9 | T-cell deficient | rag1 WISH | p53-dependent apoptosis | ||
ceylon | tbl3 | Lack of lymphoid cells | rag1 WISH | Reduced population of T cells and red blood cells | ||
grechetto | cpsf1 | Bloodless | mpx WISH | Abnormal polyadenylation of gene needed for HSC development | ||
cas003 | topbp1 | Decreased HSCs | cmyb WISH | p53-dependent apoptosis due to abnormal ATR/Chk1 activation | ||
cas002 | kri1l | Decreased HSCs | cmyb WISH | Autophagy due to accumulation of unfolded protein activation of PERK | ||
cas008 | gemin5 | Reduced myb expression | cmyb WISH | Reduced proliferation of HSCs | ||
smu07 | slc20a1b | Bloodless | βe1-globin WISH | Cell arrested in G2/M phase | ||
HSC niche | cas005 | itga4 | Decreased HSCs | cmyb WISH | Defective interaction between HSC and macrophages | |
oloca | naca | Decreased neutrophils | Sudan black B staining | Reduced survival of stromal cells |
