Fig. 6: LncRNA-HEIH/miR-194-5p/GNA13 axis regulates HCC proliferation.

GNA13 expression was inversely correlated and correlated with miR-194-5p (a) and lncRNA-HEIH (b) expression, respectively, in HCC tissues based on the TCGA database from starBase V2.0 platform. c Meta-analysis of TCGA RNA-sequencing level data for GNA13 expression in normal and tumor tissue from the GEPIA platform (LIHC; liver hepatocellular carcinoma). TPM: transcripts per kilobase per million mapped reads. d Kaplan‒Meier curves display the overall survival of patients with low and high GNA13 expression in the TCGA dataset from the GEPIA platform. e Huh7 cell lysates that were transfected with lncRNA-HEIH plasmid, silncRNA-HEIH, miR-194-5p mimic, or miR-194-5p inhibitor were employed for WB. f Similar to (e), however, Huh7 cells were transfected with the indicated siRNA and miRNA inhibitor. g UPF1 was depleted in the indicated cell lines. GNA13 proteins and mRNA were observed by WB and RT‒qPCR, respectively. The level of target mRNA detected by RT‒qPCR was normalized to that of GAPDH mRNA. The indicated cell lines transfected with GNA13 plasmid (h) or siGNA13 (i) were used to measure cell growth over a 96 h period. j Suggested model. *p ≤ 0.05; **p ≤ 0.01.