Fig. 2: Cat D negatively regulates the expression and secretion of TGFBI. | Experimental & Molecular Medicine

Fig. 2: Cat D negatively regulates the expression and secretion of TGFBI.

From: Cathepsin D promotes polarization of tumor-associated macrophages and metastasis through TGFBI-CCL20 signaling

Fig. 2

a, b Heatmap of significant proteins (a) and molecular functional GO analysis (b) based on LC‒MS/MS proteomics. c, d The protein and mRNA expression of the indicated genes in Caki and MDA-MB-231 cells. e Secretory TGFBI content in CM collected from the WT and KO mice was determined using an enzyme-linked immunosorbent assay (ELISA). f–j KO cells were transfected with a Cat D overexpression vector (OE). WT and KO cells were transfected with the same amount of an empty vector. Representative western blot images of the indicated proteins in the cells transfected with Cat D OE or empty vector (f). Protein (g) and mRNA (h) expression of EMT markers in the transfected cells. TGFB mRNA (i) and secretory (j) levels in Caki and MDA-MB-231 cancer cells. Error bars represent the ± SEM. *p < 0.01 compared with the WT cells. #p < 0.01 compared with the KO cells.

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