Fig. 5: Loss of Cat D induces the reprogramming of THP-1 polarization into an M1-like subtype. | Experimental & Molecular Medicine

Fig. 5: Loss of Cat D induces the reprogramming of THP-1 polarization into an M1-like subtype.

From: Cathepsin D promotes polarization of tumor-associated macrophages and metastasis through TGFBI-CCL20 signaling

Fig. 5

a–d THP-1 cells were cultured with either WT-CM or KO-CM from Caki cancer cells receiving rcTGFBI or iTGFBI for 2 days. CD204+ (a) and CD86+ (b) THP-1 cells were determined by FACS analysis. Protein (c) and mRNA (d) expression of the indicated genes from polarized THP-1 cells. e THP-1 macrophages were polarized with WT-CM or KO-CM from Caki cancer cells for 2 days, and then, CD163+ THP-1 (red) was observed by Immunofluorescence staining. The stained cells were counted under a fluorescence microscope at 20× magnification. IL-4- and IL-13-induced M2 macrophages were used as positive controls. Error bars represent the ± SEM. *p < 0.01 in a two-sided t test.

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