Fig. 1: Overall BOLT-seq scheme. | Experimental & Molecular Medicine

Fig. 1: Overall BOLT-seq scheme.

From: Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube

Fig. 1

Bulk transcriptOme profiling of cell Lysate in a single poT—BOLT-seq. The steps of BOLT-seq are shown schematically. Sequential reactions were carried out in a single well of a 96-well microtiter plate with no intermediate purification steps. After the final library amplification step, products are purified from each well and pooled as desired.

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