Fig. 4: Application of BOLT-seq.

a Schematic diagram of drug screening with BOLT-seq. b Uniform manifold approximation and projection (UMAP) clustering of data for 35 drugs × 2 days × three replicates (DMSO n = 9). c Top 20 significant DE genes detected in cells exposed to AMG510 or Trametinib. d Gene Set Enrichment Analysis for AMG510 and Trametinib.