Fig. 1: Ornithine aminotransferase (OAT) expression is increased in the lungs of patients with idiopathic pulmonary fibrosis (IPF). | Experimental & Molecular Medicine

Fig. 1: Ornithine aminotransferase (OAT) expression is increased in the lungs of patients with idiopathic pulmonary fibrosis (IPF).

From: Role of lung ornithine aminotransferase in idiopathic pulmonary fibrosis: regulation of mitochondrial ROS generation and TGF-β1 activity

Fig. 1

a Immunohistochemical staining for OAT in the lungs of control subjects compared to patients with IPF. Original magnification 100×; inset magnification 1000×. The arrowheads indicate alveolar epithelial cells (AECs) in the normal control tissues, hyperplastic AECs in mildly fibrotic tissues and metaplastic bronchiolar epithelial cells in parenchymal scars, and arrows indicate interstitial fibroblasts. b OAT expression in lung tissues was measured by immunoblotting. OAT bands were quantified using densitometry. *p < 0.05 versus the control subjects. An anti-OAT antibody was used to immunoblot equal quantities of proteins obtained from lung lysates. The blots were stripped and reprobed with an anti-β-actin antibody. Lung tissues were obtained from patients with IPF and control subjects who underwent thoracic surgery. c. OAT was not expressed in inflammatory cells in bronchoalveolar lavage fluid. Magnification, 1000×. d OAT expression was induced by BLM. BLM was administered into the tracheas of wild-type mice that were subsequently sacrificed on Day 21. Strong OAT expression was observed in fibrotic areas. Magnification, 100×; inset magnification, 1000×. OAT was not expressed in inflammatory cells. Magnification, 1000×.

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