Fig. 7: The OAT inhibitor L-canaline attenuates BLM-induced lung inflammation and fibrosis in a mouse model. | Experimental & Molecular Medicine

Fig. 7: The OAT inhibitor L-canaline attenuates BLM-induced lung inflammation and fibrosis in a mouse model.

From: Role of lung ornithine aminotransferase in idiopathic pulmonary fibrosis: regulation of mitochondrial ROS generation and TGF-β1 activity

Fig. 7

a H&E staining of mouse lungs after 100 nM L-canaline was intranasally administered on Days 8–12. Lung samples were collected on Day 21. Magnification, 100×. b Cell counts in BALF collected on Day 21. Differences between BALF cell counts were analyzed based on 500 cells stained with Diff-Quik (n = 8 per group). c Masson’s trichrome staining. Magnification, 100×. d Lung fibrosis as quantified using the Ashcroft score (n = 8 per group). e Collagen measurements from hydroxyproline assays of control and BLM-treated mouse lungs, with and without L-canaline (n = 8 per group). f Active TGF-β1 and g OAT levels in control and BLM-treated mouse lungs, with and without L-canaline (n = 8 per group). Active TGF-β1 and OAT levels in lung lysates were quantified using ELISAs. The data are expressed as the means ± SEMs. *p < 0.05 versus the BLM-treated group.

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