Fig. 3: Acetate uptake in normal mouse liver tissue and cancer cells and its relationship with cell growth. | Experimental & Molecular Medicine

Fig. 3: Acetate uptake in normal mouse liver tissue and cancer cells and its relationship with cell growth.

From: Acetyl-CoA synthetase 2 contributes to a better prognosis for liver cancer by switching acetate-glucose metabolism

Fig. 3

a Glutamate isotopomer distribution in normal liver tissue of mice orally administered U-13C6-glucose (left) or 1,2-13C2-acetate (right) (both 5 g/kg), as measured via liquid chromatography–mass spectrometry (LC–MS). The sampling times for the tracers differ to reflect the kinetic differences in absorption between the two tracers. b Uptake of 1,2-13C2-acetate (left, 0.2 mM) or U-13C6-glucose (right, 5 mM) by various liver cell lines measured by nuclear magnetic resonance (NMR) intensities of the remaining tracers in the media compared with their initial intensities (leftmost for each). c ACSS2 expression in various liver cancer cells. Western blot analysis of ACSS2 expression in Huh-7, PLC/PRF/5, Hep3B, HepG2, and Huh-6 cells and real-time quantitative polymerase chain reaction (qRT–PCR) analysis of ACSS2 expression in Hep3B and HepG2 cells. d Liver cancer cell growth was measured by a colorimetric cell viability assay in the presence of the indicated concentrations of acetate and glucose. e Clonogenic and scratch wound healing assays were performed for Hep3B and HepG2 cells. f Cell survival of shACSS2 HepG2 and OE-ACSS2 Hep3B cells determined by clonogenic assay after 14 days and cell proliferation of shACSS2 HepG2 and OE-ACSS2 Hep3B cells after 48 h. N.S.: not significant; *p < 0.05, **p < 0.01, **p < 0.001 (n = 3) according to Student’s t test with standard deviations.

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