Fig. 2: Activation of AGE-RAGE signaling enhances the osteogenic differentiation and mineralization of meniscal cells. | Experimental & Molecular Medicine

Fig. 2: Activation of AGE-RAGE signaling enhances the osteogenic differentiation and mineralization of meniscal cells.

From: Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Fig. 2

a Human primary meniscal cells were treated with the indicated drugs during osteogenic induction, and Alizarin Red was used to detect osteogenic differentiation. b Quantification of Alizarin Red staining; n = 3. c, e Three-dimensional micro-CT images of the calcification area in the meniscal region and merged images with the knee joints in different mouse groups. Scale bar: 1 mm. d Quantification of the calcification volume; n = 5. f Quantification of the calcification volume; n = 4. O.I., osteogenic induction (21 days). The data are expressed as the mean ± SD; *P < 0.05; **P < 0.01; and ***P < 0.001. One-way ANOVA was used for comparisons.

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