Fig. 3: The PI3K-AKT-mTOR signaling pathway is activated by AGE treatment and induces calcification. | Experimental & Molecular Medicine

Fig. 3: The PI3K-AKT-mTOR signaling pathway is activated by AGE treatment and induces calcification.

From: Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Fig. 3

a Enrichment plots from GSEA showing pathways enriched in the degenerated meniscus. b Venn diagram showing pathways downstream of AGE-RAGE and pathways enriched in the degenerated meniscus. c At different times during osteogenic induction, human primary meniscal cell lysates were analyzed via WB for the indicated proteins. d Quantification of the WB data; n = 3. e Human primary meniscal cells were treated with the indicated drugs during osteogenic induction; cell lysates were analyzed via WB for the indicated proteins. f Quantification of the WB data; n = 3. O.I. osteogenic induction, which means 21 days of induction when the induction time is not indicated. The data are expressed as the mean ± SD; *P < 0.05; **P < 0.01; and ***P < 0.001. One-way ANOVA was used for comparisons.

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