Fig. 5: A positive feedback loop involving mTOR and ATF4 promotes osteogenesis in meniscal cells. | Experimental & Molecular Medicine

Fig. 5: A positive feedback loop involving mTOR and ATF4 promotes osteogenesis in meniscal cells.

From: Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Fig. 5

a Human primary meniscal cells were treated with the indicated drugs or with the ATF4 KD lentivirus during osteogenic induction; cell lysates were analyzed via WB for the indicated proteins. b Human primary meniscal cells were treated with rapamycin or an ATF4 KD lentivirus (LV) during osteogenic induction; cell lysates were analyzed via qRT‒PCR for the indicated mRNAs; n = 3. c, d Human primary meniscal cells were treated with the indicated drugs or with the ATF4 KD lentivirus (LV) during osteogenic induction; Alizarin Red staining was used to detect osteogenic differentiation. e, g Quantification of the WB data in (a); n = 3. f, h Quantification of Alizarin Red staining; n = 3. O.I., osteogenic induction (21 days). The data are expressed as the mean ± SD; *P < 0.05; **P < 0.01; and ***P < 0.001. Student’s t test (b) and one-way ANOVA (e–h) were used for comparisons between two groups and multiple groups.

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