Fig. 7: ATF4 induces arginine accumulation to activate mTOR in meniscal cells. | Experimental & Molecular Medicine

Fig. 7: ATF4 induces arginine accumulation to activate mTOR in meniscal cells.

From: Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Fig. 7

a A heatmap was generated using mass spectrometry data. The position indicated by the arrow is the arginine, n = 3. b Human primary meniscal cells were treated with the indicated drugs during osteogenic induction; Alizarin Red staining was used to detect osteogenic differentiation. c Quantification of Alizarin Red staining, n = 3. d Human primary meniscal cells were treated with the indicated drugs during osteogenic induction; cell lysates were analyzed via WB for the indicated proteins. e Quantification of the WB data; n = 3. f–h Human primary meniscal cells were treated with the indicated drugs during osteogenic induction; relative quantification of the intracellular arginine concentration was performed via ELISA; n = 3. O.I., osteogenic induction (21 days). The data are expressed as the mean ± SD; *P < 0.05; **P < 0.01; and ***P < 0.001. One-way ANOVA was used for comparisons.

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