Fig. 3: Radial glial-like cells deviate from their normal developmental course and give rise to CN.

a snRNA-seq analysis of paired CN tumor and normal brain samples. b Cell-specific gene markers for each identified cell type are shown. FGFR3 can be detected in both tumor and radial glial cells. c Confirmation of overexpression of FGFR3, the PI3K-AKT1 pathway, and radial glial cell markers in tumor cell clusters consistent with the bulk RNA-seq analysis. d Pseudotime analysis of tumor cells, neurons, oligodendrocytes, and radial glial/astrocyte-like cells showing tumor cells positioned between neurons and radial glial/astrocyte-like cells. e Genes expressed in both neurons and radial glial/astrocyte-like cells but not in tumor cells. f PCA plot showing that despite being identified as the same cell type, radial glial/astrocyte-like cells from tumor and normal samples show some differences. g Heatmap of differentially expressed genes in normal and tumor sample radial glial/astrocyte-like cells.