Fig. 2: BH4 metabolism protects pulmonary cells from radiation-induced damage. | Experimental & Molecular Medicine

Fig. 2: BH4 metabolism protects pulmonary cells from radiation-induced damage.

From: Tetrahydrobiopterin metabolism attenuates ROS generation and radiosensitivity through LDHA S-nitrosylation: novel insight into radiogenic lung injury

Fig. 2

a HELF cells were infected with the control adenovirus or GCH1 adenovirus and subsequently irradiated. Cell viability was measured by a CCK-8 assay at 24, 48, and 72 h after irradiation. The effect of GCH1 overexpression on the survival of (b) HELF and (c) BEAS-2B cells was evaluated. HELF and BEAS-2B cells were treated with BH4 or PBS and subsequently irradiated. d, e The clonogenic survival of the two cell lines was measured. f, g HELF and BEAS-2B cells were subjected to LDH release assays. *P < 0.05 and **P < 0.01 compared with the control group.

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