Fig. 6: BH4 restores protein S-nitrosylation after irradiation.

a Experimental design of the protein S-nitrosylation analysis. b (Left) Number of dysregulated S-nitrosylation sites and proteins with differential S-nitrosylation sites in mouse lungs exposed to 0 and 20 Gy of radiation. (Right) Number of dysregulated S-nitrosylation sites and proteins with differential S-nitrosylation sites between control (PBS)- and BH4-treated irradiated mouse lung tissues. c BH4 restored protein S-nitrosylation in HELF cells. Protein S-nitrosylation was determined by a biotin switch assay. The sample without ascorbate was representative of endogenous biotinylated protein levels. d IodoTMT-based quantitative protein S-nitrosylation analysis was used to identify S-nitrosylated proteins that were dysregulated in lung tissues. Key modified sites were identified. e Venn diagram showing proteins with S-nitrosylation in the two groups. f DAVID functional clustering and literature curation were performed to identify pathways targeted by protein S-nitrosylation.