Fig. 3: Propranolol inhibits epinephrine-mediated PLAGL2 expression and HCC progression. | Experimental & Molecular Medicine

Fig. 3: Propranolol inhibits epinephrine-mediated PLAGL2 expression and HCC progression.

From: Stress-induced epinephrine promotes hepatocellular carcinoma progression via the USP10-PLAGL2 signaling loop

Fig. 3

a, b HCC cells were treated with Epi (100 pM) for 48 h and then treated with propranolol (10 μM) for 24 h. The protein expression of PLAGL2 and vimentin was analyzed by Western blot analysis. c–e Effects of propranolol on the migration of HCC cells. Representative images of HCC cells in the Transwell (c) and wound healing assays (d, e). f, g Statistical analysis of the wound healing assay results (n = 3). h Tumor growth of Hepa1-6 cells in mice treated with Epi (2 mg/kg/d, s.c.) or propranolol (2 mg/kg/d, i.p.). i, j Images and statistical analysis of tumor weights in model mice treated with Epi and propranolol (n = 6). k, l Western blot and quantitative analyses of the protein levels of EMT-related factors (N-cadherin and vimentin) and PLAGL2 in the tumors of mice treated with Epi and propranolol. m, n IHC and quantitative analyses of PLAGL2, Ki67, vimentin, and N-cadherin expression in the tumors of mice treated with Epi or propranolol. The data are presented as the mean ± SD and were analyzed by two-tailed Student’s t tests. *p < 0.05, **p < 0.01, ***p < 0.001. Pro propranolol.

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