Fig. 4: PLAGL2 knockdown inhibits Epi-induced HCC progression in vitro and in vivo. | Experimental & Molecular Medicine

Fig. 4: PLAGL2 knockdown inhibits Epi-induced HCC progression in vitro and in vivo.

From: Stress-induced epinephrine promotes hepatocellular carcinoma progression via the USP10-PLAGL2 signaling loop

Fig. 4

a, b PLAGL2-knockdown and PLAGL2-overexpressing HCC cells were treated with or without Epi (100 pM) for 48 h, and Western blot analysis of the protein expression of PLAGL2 and Vimentin was performed. c–f Effects of Epi on the migration of PLAGL2-knockdown or -overexpressing HCC cells. Representative images of HCC cells in the wound healing (c, d) and Transwell (e, f) assays. g Tumor growth of shCtrl and shPLAGL2 Hepa1-6 cells in mice treated with or without Epi (2 mg/kg/d, s.c.). h, i Images and statistical analysis of tumor weights in the shCtrl and shPLAGL2 Hepa1-6 groups treated with or without Epi. j, k Western blot and quantitative analyses of the protein levels of EMT-related factors (N-cadherin and vimentin) and PLAGL2 in shCtrl and shPLAGL2 Hepa1-6 tumors from mice treated with or without Epi. l, m IHC and quantitative analyses of PLAGL2, Ki67, vimentin, and N-cadherin expression in shCtrl and shPLAGL2 Hepa1-6 tumors in mice treated with Epi. Analyses were performed by two-tailed Student’s t tests. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Epi epinephrine.

Back to article page