Fig. 8: Epinephrine induces the ADRB2-c-Myc axis to activate USP10 transcription and promote HCC progression.

a Model of Epi-induced USP10-PLAGL2 signaling loop activation via ADRB2-c-Myc. b, c HCC cells were treated with ICI118, 551 (10 μM) or Epi (100 pM) for 48 h, after which Western blot analysis was performed to measure the protein levels of ADRB2, c-Myc, USP10, and PLAGL2. d, e HCC cells were transfected with c-Myc siRNA (si465) for 24 h and treated with Epi (100 pM) for 48 h, after which Western blot analysis was performed to determine the protein levels of c-Myc, USP10 and PLAGL2. f, g HCC cells were transfected with USP10 siRNA (si1756) for 24 h and treated with Epi (100 pM) for 48 h, after which Western blot analysis was performed to determine the protein levels of USP10 and PLAGL2. h, i HCC cells were treated with the USP10-specific antagonist Spautin-1 (10 μM) or Epi (100 pM) for 48 h, after which Western blot analysis was performed to measure the protein levels of USP10 and PLAGL2. j–m Representative images of HCC cells treated with Spautin-1 (10 μM) or Epi (100 pM) for 24 h or 96 h and examined by a wound healing assay. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Epi epinephrine.