Fig. 5: Psoriatic skin inflammation and the increase in intestinal permeability are attenuated in eosinophil-deficient mice. | Experimental & Molecular Medicine

Fig. 5: Psoriatic skin inflammation and the increase in intestinal permeability are attenuated in eosinophil-deficient mice.

From: TLR7-dependent eosinophil degranulation links psoriatic skin inflammation to small intestinal inflammatory changes in mice

Fig. 5

a Schematic of the adoptive transfer (Tf) of bone marrow-derived eosinophils (BMEOs). WT, wild-type; ΔEO, ΔdblGATA. (b) Hematoxylin and eosin-stained skin. Scale bars, 124.5 μm. c Epidermal thickness. Three distinct regions of each section were analyzed. d Transepidermal water loss (TEWL) (n = 9 or 10/group). *P < 0.05, ***P < 0.001, and ****P < 0.0001, WT IMQ vs. ΔEO IMQ; #P < 0.05, ##P < 0.01, ΔEO IMQ + BMEO Tf vs. ΔEO IMQ. e Quantitative PCR analysis of the skin. f Serum concentrations of eosinophil peroxidase (EPX) and calprotectin. g Stool concentrations of EPX and calprotectin. h Quantitative PCR analysis of the small intestine (SI). Occludin and mucin 2 (green) immunofluorescence staining (i) and quantification (j) in the SI. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; blue). The fluorescence intensity of each section was analyzed. Scale bars, 62.3 μm. The data are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; one-way ANOVA with Tukey’s multiple comparisons (c, e, f, and h); Kruskal–Wallis test with Dunn’s multiple comparisons (Il23 in e, g, and Tnf and Tjp1 in h); two-way ANOVA with Bonferroni’s multiple comparisons (d); or unpaired t test (j).

Back to article page