Fig. 6: Eosinophil degranulation and inflammatory changes in the small intestine in mice with psoriatic skin inflammation are induced in a TLR7-dependent manner.

a Representative images of blood perfusion on the dorsal skin of mice. IMQ, imiquimod. The color density indicates the level of microcirculation. IMQ concentrations in the serum (b) and intestine (c). ND, not detected. d Absolute number of eosinophils in naïve mice. e, g Schematic of eosinophil sorting from the small intestine (SI). WT, wild-type. f, h Representative flow cytometry plots (left) and percentages (right) of sorted eosinophils stained with Annexin V⁺ or 7-aminoactinomycin D (7-AAD)⁺ after being cultured with IMQ. i Hematoxylin and eosin-stained skin. Scale bars, 124.5 μm. j Epidermal thickness. Three distinct regions of each section were analyzed. k Quantitative PCR analysis of the skin. l Weight change (n = 4 or 5/group). m Representative flow cytometry plots (left) and percentages (right) of SiglecF⁺, CD63⁺, and SIRPα⁺ eosinophils in the SI. n Sequencing of the stool microbiota of the SI (n = 3). o Quantitative PCR analysis of the SI. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, and ****P < 0.0001 according to the Kruskal–Wallis test with Dunn’s multiple comparisons (b); one-way ANOVA with Tukey’s multiple comparisons (c, d, f, and h); Mann–Whitney test (j, k, and o); unpaired t test (Il23 in k and Tnf, S100a8, and Ocln in o); or two-way ANOVA with Bonferroni’s multiple comparisons (l).