Fig. 5: RBM15 directly regulates genes involved in serine and glycine metabolism in BC patients. | Experimental & Molecular Medicine

Fig. 5: RBM15 directly regulates genes involved in serine and glycine metabolism in BC patients.

From: The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism

Fig. 5

a RIP-seq data (GSE73893) were processed for sequence distribution. b Comparison of gene expression data and RIP-seq data using a Venn diagram. c IPA analysis with comparison data. d UCSC genome browser view of RIP-seq reads mapped to PHGDH, PSAT1, PSPH, and SHMT2. e Significantly enriched RNA motifs among the RIP-seq data. f Sequence alignment of the PHGDH, PSAT1, PSPH, and SHMT2 genomic loci based on the RBM15 binding motif. g RIP analyses of MDA-MB-231 and Hs 578 T cells were performed using the indicated antibodies. Next, qRT‒PCR analysis was performed using primers against the PHGDH, PSAT1, PSPH, and SHMT2 mRNAs. h, i MDA-MB-231 cells were infected with shRBM15 or shGFP (h) and pCDH-RBM15 or empty vector (i). After infection, the cells were treated with DMSO or actinomycin D (Act D) and harvested at the indicated time points. Total RNA was extracted from the indicated cells and analyzed by qRT‒PCR with the indicated primers. The RNA level is designated the RNA half-life. All results are expressed as the means ± standard deviations (SDs) from replicates (*p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.001).

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