Fig. 7: SSP gene regulation by RBM15 is dependent on the m6A writer METTL3/14.
a MDA-MB-231 and Hs 578T cells were transfected with the indicated shRNAs, and the cell lysates were subjected to WB analysis with the indicated antibodies. b The infected cells were treated with DMSO or actinomycin D (Act D) and harvested at the indicated time points for qRT‒PCR. c, d MDA-MB-231 and Hs 578T cells were transfected with the indicated shRNAs and Flag-tagged RBM15 or empty vector. The infected cells were analyzed by WB (c) or qRT‒PCR (d) using the indicated antibodies and primers, respectively. e–g Diagram of the RBM15 mutant construct (e). MDA-MB-231 and Hs 578T cells were transfected with Flag-tagged RBM15 or empty vector and analyzed by WB using the indicated antibodies (f). The infected cells were treated with DMSO or actinomycin D (Act D) and harvested at the indicated time points for qRT‒PCR with the indicated primers (g). The data are expressed as the means ± SDs of triplicate samples (*p < 0.05, **p < 0.01, and ***p < 0.005).
