Fig. 1: Hypoxia increases miR-214-3p biogenesis, which, in turn, inhibits PlGF expression in trophoblasts. | Experimental & Molecular Medicine

Fig. 1: Hypoxia increases miR-214-3p biogenesis, which, in turn, inhibits PlGF expression in trophoblasts.

From: Alleviation of preeclampsia-like symptoms through PlGF and eNOS regulation by hypoxia- and NF-κB-responsive miR-214-3p deletion

Fig. 1

a HTR-8/SVneo cells were transfected with empty (mock) vector, pcDNA3.1-mutant HIF1A (mHIF1A), pGL3-PlGF promoter, psiCHECK-2-PlGF 3′-UTR, or Dicer siRNA (siDicer) and subjected to normoxia or hypoxia for 24 h. PlGF mRNA and protein levels were determined using qRT‒PCR and western blotting, respectively (n = 4). PlGF promoter and mRNA 3′-UTR activities were determined in cell lysates using a luciferase activity assay (n = 4). b The expression profile of miRNAs was assessed in HTR-8/SVneo cells exposed to normoxia or hypoxia for 24 h using a microarray (n = 3). c The expression of miR-214-3p and PlGF in HTR-8/SVneo cells exposed to normoxia or hypoxia was determined using qRT‒PCR and western blotting, respectively (n = 4). d HTR-8/SVneo cells were transfected with miR-214-3p mimics (miR-214-3p-M) for 24 h. PlGF levels were determined using western blotting (n = 4). The data are presented as the mean ± SEM. Statistical significance was determined using one-way ANOVA, followed by the Holm–Sidak’s multiple comparisons test. ns not significant.

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