Fig. 6: MiR-214-3p deficiency prevents PE-like characteristics in hypoxic pregnant mice. | Experimental & Molecular Medicine

Fig. 6: MiR-214-3p deficiency prevents PE-like characteristics in hypoxic pregnant mice.

From: Alleviation of preeclampsia-like symptoms through PlGF and eNOS regulation by hypoxia- and NF-κB-responsive miR-214-3p deletion

Fig. 6

Pregnant WT and miR-214-3p KO mice were exposed to normoxia (N) or hypoxia (H) from GD 9.5 to GD 17.5. a Twist1 mRNA levels were determined in placental tissues using qRT‒PCR (n = 8). b MiR-214-3p levels were determined in placental tissues and maternal sera using qRT‒PCR (n = 8). c PlGF mRNA levels were determined in placental tissues using qRT‒PCR (n = 8). d–f Serum levels of PlGF, VEGF, and sFlt-1 were quantified using ELISA kits (n = 8). g The sFlt-1/PlGF ratio was calculated from the serum concentration of each protein (n = 8). h, i Levels of eNOS and NO were determined in mouse aortic vessels using western blotting and DAF-FM-based fluorescence microscopy, respectively (n = 8). j The vasorelaxation responses of mouse aortic rings to Ach were determined using myography (n = 5). The data are presented as the mean ± SEM. Statistical significance was determined using multiple t tests (j) or one-way ANOVA (a–g, i), followed by the Holm–Sidak’s multiple comparisons test. ns, not significant.

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