Fig. 8: MiR-214-3p deficiency alleviates spiral artery remodeling in hypoxic pregnant mice. | Experimental & Molecular Medicine

Fig. 8: MiR-214-3p deficiency alleviates spiral artery remodeling in hypoxic pregnant mice.

From: Alleviation of preeclampsia-like symptoms through PlGF and eNOS regulation by hypoxia- and NF-κB-responsive miR-214-3p deletion

Fig. 8

Pregnant WT and miR-214-3p KO mice were treated as described in Fig. 6. a Representative images of placental tissues stained with H&E. Scale bar = 100 μm. SA spiral artery. b The diameter of the spiral arteries was measured using ImageJ and is expressed as the average value of five random vessels in each placental tissue section (n = 8 litters). c, f Representative images of placental tissues stained with DAPI (nuclear marker) and antibodies against cytokeratin (CK, a trophoblast marker), CD31 (an endothelial cell marker), or α‐smooth muscle actin (α-SMA, a smooth muscle cell marker) and visualized using confocal microscopy. Scale bar = 100 μm. Areas of CK+ trophoblasts (d), CD31+ endothelial cells (e), and α-SMA+ smooth muscle cells (g) around or within vessels were determined using ZEN software and are expressed as the mean value of five random vessels in each placental tissue section (n = 8 litters). The data are presented as the mean ± SEM. Statistical significance was determined using one-way ANOVA, followed by the Holm–Sidak’s multiple comparisons test. ns not significant.

Back to article page