Fig. 4: Vhl cKO alleviates T1DM partially via RegIIIγ.

a, b RegIIIγ IF staining and RegIIIγ mRNA expression were analyzed in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice. c Serum RegIIIγ levels were measured in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice. d Fasting blood glucose levels were determined in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction (n = 5). e GTTs were performed on the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction (n = 5). f Serum insulin levels were measured in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice after STZ induction (n = 5). g GSIS was assessed in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice after STZ induction (n = 5). h Representative images of pancreatic islets from the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction were subjected to HE staining and insulin immunostaining. i, k, l Islets of the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction were subjected to immunohistochemical (IHC) staining for BAX and PCNA. j Insulin mRNA expression was analyzed in the pancreatic islets of the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction (n = 3). m The islet equivalent quantity (IEQ) was calculated for the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice subjected to STZ induction (n = 5). n Changes in body weight were measured in the control, Vhl cKO, RegIIIγ cKO, and Vhl-RegIIIγ cKO mice after STZ induction (n = 5). All data are presented as the mean ± SEM, and p values were analyzed by one-way ANOVA for (b–d, f, j–m) and two-way ANOVA for (e, g, n). *p < 0.05, **p < 0.01, ***p < 0.001. All the data are representative of two to three independent experiments. The data were obtained from male mice.