Fig. 4: Function of YAP1 in BLM-induced lung injury and senescence.

a Representative images showing Sftpc (red), Aqp5 (green), and nuclei (blue) in frozen lung sections from WT and YAP1-cKO mice. TUNEL assay of apoptosis, with TUNEL-positive cells indicated by green fluorescence and nuclei marked with DAPI. Scale bars, 20 μm. b Images of HE staining in tissues from WT and YAP1-cKO mice treated with saline or BLM. Scale bars, 50 μm. c Western blot analysis of p21 protein expression in WT and YAP1-cKO mice treated with saline or BLM; n = 6. d qRT‒PCR analysis of the mRNA expression of IL-6, IL-8, and CXCL1; n = 6. e SA-β-gal and IHC staining in lung sections from WT and YAP1-cKO mice treated with saline or BLM. Scale bars, 50 μm. f Representative images of frozen lung sections from WT and YAP1-cKI mice, with fluorescence indicating the following: Sftpc (red), Aqp5 (green), and nuclei (blue). TUNEL-positive cells are indicated by green fluorescence, and nuclei are indicated by blue fluorescence. Scale bars, 20 μm. g Images of HE staining in tissues from WT and YAP1-cKI mice treated with saline or BLM. Scale bars, 50 μm. h Western blot analysis of p21 protein expression in WT and YAP1-cKI mice treated with saline or BLM; n = 6. i qRT‒PCR analysis of the mRNA expression of IL-6, IL-8, and CXCL1; n = 6. j SA-β-gal and IHC staining in lung sections from WT and YAP1-cKI mice treated with saline or BLM. Scale bars, 50 μm. The data are presented as the means ± SEMs. **P < 0.01.