Fig. 5: YAP1/TEAD1 directly targets Prdx3. | Experimental & Molecular Medicine

Fig. 5: YAP1/TEAD1 directly targets Prdx3.

From: YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis

Fig. 5

a Proteomic analysis showing that signaling pathways were modulated by YAP1. b ChIP sequencing analysis results demonstrating the signaling pathways regulated by TEAD1. c, d Heatmap showing the expression of overlapping significantly altered genes identified by integrating the proteomics and ChIP sequencing data. e, f Western blot and qRT‒PCR analyses of the expression of Prdx3 in MLE-12 cells transfected with the YAP1 plasmid or si-YAP1, n = 3. g ChIP assay showing the relative ChIP product abundances in MLE-12 cells transfected with the YAP1 or TEAD1 plasmid; n = 3. h Relative luciferase activity was measured in MLE-12 cells transfected with various fragments of the Prdx3 promoter; n = 3. i Western blot showing the expression levels of Prdx3 in YAP1-overexpressing MLE-12 cells treated with verteporfin or expressing YAP1S94A; n = 3. The data are presented as the means ± SEMs. ** P < 0.01.

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