Fig. 6: Silencing of Prdx3 abolishes the effects of YAP1 on senescence and mitochondrial dysfunction.
a Western blot analysis of p16 and p21 expression; n = 6. b SA-β-gal staining to evaluate senescence in MLE-12 cells transfected with the YAP1 overexpression plasmid or si-Prdx3 after BLM treatment; n = 6. Scale bars, 50 μm. The cell size was determined by confocal microscopy, and nuclei were stained with Hoechst 33342 (blue). Scale bars, 10 μm. c Western blot results showing the protein expression of Bax and Cyt-C in MLE-12 cells transfected with the YAP1 overexpression plasmid or si-Prdx3 after BLM treatment; n = 3. d Immunofluorescence staining of γH2AX in MLE-12 cells with YAP1 overexpression or Prdx3 silencing following BLM induction. Scale bars, 20 μm. e The protein levels of Drp1 and Mfn2 were measured by western blotting, n = 5. f Cytosolic and mitochondrial ROS levels in Prdx3-overexpressing MLE-12 cells after BLM administration were measured using a DCFH-DA probe and MitoSOX. Scale bars, 50 μm. g Representative images of MLE-12 cells stained with TMRM (red) and DAPI (nuclei; blue). Scale bars, 10 μm. h Representative images acquired by confocal microscopy showing morphological changes in mitochondria. Scale bars, 10 μm. i The O2 consumption rate was assessed using a Seahorse mitochondrial stress test. The data are presented as the means ± SEMs. *P < 0.05, **P < 0.01.
