Fig. 7: IL-3399–270 and IL-33109–270 regulate OIR-induced retinal EC proliferation. | Experimental & Molecular Medicine

Fig. 7: IL-3399–270 and IL-33109–270 regulate OIR-induced retinal EC proliferation.

From: A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy

Fig. 7

a Schematic diagram showing the murine OIR model. C57BL/6 mouse pups with dams were exposed to 75% oxygen from P7 to P12 and returned to the room at P12. The P12–P17 phase is the neovascular phase. b C57BL/6 mouse pups with dams were exposed to 75% oxygen from P7 to P12 and returned to the room at P12. The mouse pups were subjected to intraperitoneal (i.p.) injection with PBS, recombinant IL-3395–270, IL-3399–270 or IL-33109–270 at P12, P13, and P14. At P15, the eyes were enucleated, the retinas were isolated, and cryosections were prepared and stained with anti-HA tag antibodies. c IL-33+/+ and IL-33−/− pups were subjected to i.p. injection with PBS or the mature forms of IL-33, and at P15, the retinas were isolated and fixed, and cross-sections were made and subjected to immunofluorescence staining for CD31 and Ki67. d Retinal EC proliferation was measured by counting CD31- and Ki67-positive cells that extended anteriorly to the inner limiting membrane per section (n = 6 eyes, 3 sections/eye). The scale bar represents 20 μm in (b, c).

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