Fig. 8: IL-3399–270 and IL-33109–270 regulate OIR-induced retinal neovascularization. | Experimental & Molecular Medicine

Fig. 8: IL-3399–270 and IL-33109–270 regulate OIR-induced retinal neovascularization.

From: A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy

Fig. 8

a, c, e IL-33+/+ and IL-33−/− pups were subjected to i.p. injection with PBS, recombinant IL-3395–270, IL-3399–270 or IL-33109–270 at P12, P13, and P14. At P17, the eyes were enucleated, and the retinas were isolated, fixed, and stained with isolectin B4. Flat mounts were prepared and examined for retinal neovascularization (a), avascular areas (c), and endothelial tip cell formation (e). Neovascularization is highlighted in red in (a). The bar graphs represent the quantitative analysis of neovascularization (b), avascular areas (d), and number of sprouts/100 mm vessel length (f). There were n = 8 (b, d, f) biologically independent eyes per group, and the data are expressed as the mean ± SD. The scale bars represent 500 μm in (a, c), 20 μm in (e) in the upper row, and 50 μm in (e) in the lower row.

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