Fig. 2: Enrichment analysis and screening of hub genes using key modular rows associated with sample traits.

a GO (BP) enrichment analysis of purple module genes. b GO (BP) enrichment analysis of shared DEGs and purple module genes. c, d GO and KEGG enrichment analysis of the shared DEGs, purple module genes, and neutrophil-related genes. e PPI network construction and selection of hub genes, where core modular genes of the MCODE screen are located in the middle and inner circles, and hub genes of the cytoHubba screen are located in the inner circle. f GSEA was used for the final screening of hub genes. NES represents the average of the enrichment scores obtained for each pathway, and FDR determines the rate of false-positive findings that may be contained therein. Absolute NES values were negatively correlated with FDR, indicating high enrichment and reliable results. g t-SNE visualization showing the outcomes of cell type annotation. The expression levels of all genes expressed by cells in each cluster were quantified and ranked, followed by the annotation of cell types using various methods. h The expression of LY86 in various cell types was examined using single-cell transcriptome data associated with the formation of unstable carotid plaques. i Macrophage subpopulations. j Expression of LY86 in different subpopulations. k Presentation of the three cell types in the pseudotime analysis trajectory. l Pseudotime order in the pseudotime analysis trajectory. m The differentiation of LY86 in the pseudotime analysis trajectory.