Fig. 5: MD-1 induces the formation of NETs via the Toll-like receptor pathway. | Experimental & Molecular Medicine

Fig. 5: MD-1 induces the formation of NETs via the Toll-like receptor pathway.

From: Neutrophil extracellular traps mediate the crosstalk between plaque microenvironment and unstable carotid plaque formation

Fig. 5

a, b Probing the temporal gradient of induction of NETs upon addition of pathway inhibitors (scale bar = 100 μm). c Inhibition of TLR4, MyD88, IRAK1-4, and TRAF6 with specific inhibitors abrogated MD-1-induced TLR4 expression as well as the phosphorylation of IRAK1/IRAK4, p38 MAPK, and ERK1/2; inhibition of NADPH oxidase with DPI did not affect the phosphorylation of p38 MAPK or ERK1/2. t total value; p phosphorylation value. β-Actin was used as an internal control.

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