Fig. 7: NETs activate the NF-κB signaling pathway to promote intraplaque neovascularization. | Experimental & Molecular Medicine

Fig. 7: NETs activate the NF-κB signaling pathway to promote intraplaque neovascularization.

From: Neutrophil extracellular traps mediate the crosstalk between plaque microenvironment and unstable carotid plaque formation

Fig. 7

a HUVECs were screened for relevant DEGs after NETs stimulation, and the results were visualized in the form of a volcano plot and heatmap. b Intersection of deg and deg (NETs st. HUVEC) related genes. c GO and KEGG analyses. d Tube formation assay with HUVECs treated as described above. e Representative images of immunofluorescence staining visible in unstable plaques of the middle carotid artery and within stable plaques with CD31 (red) showing intraplaque neovascularization. The MVD was significantly different between the two groups. (Scale bar = 100 μm). f Western blot showing the expression of VCAM1, ICAM1, MMP14, p-NF-κB, t-NF-κB, VEGFA, and IL6 in HUVECs under different conditions. g Expression of VCAM1, ICAM1, MMP14, VEGFA and IL6 in tissues. t total value; p phosphorylation value. β-Actin was used as an internal control.

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