Fig. 1: UNI418 specifically targets PIP5K1 and PIKfyve.
From: A dual inhibitor of PIP5K1C and PIKfyve prevents SARS-CoV-2 entry into cells
a Chemical structure of UNI418. The 4-quinazolinamine core is highlighted in green. b Table of the kinase profiling analysis. Inhibition of several kinases was determined at a 1 μM concentration of UNI418 and analyzed by the SelectScreen Kinase Profiling Service (Thermo Fisher Scientific). c Cellular thermal shift assay. Ectopically expressed GFP-PIP5K1C and GFP-PIKfyve were heated to the indicated temperatures with/without UNI418, and the presence of protein in the soluble fraction was analyzed by Western blot analysis.
