Fig. 2: UNI418 inhibits autophagy.
From: A dual inhibitor of PIP5K1C and PIKfyve prevents SARS-CoV-2 entry into cells
a Western blot analysis of p62, LC3B-I, LC3B-II and GAPDH in the presence of UNC3230, apilimod and UNI418. b Immunofluorescence staining with LC3B (green) and p62 (red) antibodies upon treatment with UNC3230, apilimod and UNI418, and images within the white rectangular boxes are magnified on the right side. Scale bar, 20 µm. c The area of fluorescence intensity from immunofluorescence images (b) was quantified after UNC3230, apilimod and UNI418 treatments. d, e Quantification of autophagic flux under normal conditions and under HBSS-induced starvation conditions with or without UNI418 treatment. RPE1 cells were treated with UNI418 (0.5 µM or 1 µM) for 2 h in the presence or absence of a lysosomal inhibitor (bafilomycin A1, BFA). Representative Western blot images (d) and the graph showing the average of 4 independent experiments (e). The error bars indicate the S.D. Statistical analysis was performed using two-way ANOVA; *p < 0.05, ***p < 0.001.
