Fig. 3: Single-cell profiling of heart-specific Smc3-knockout embryonic cardiac tissue at E12.5.
From: SMC3 contributes to heart development by regulating super-enhancer associated genes
a Schematic of single-nucleus RNA-seq (snRNA-seq). Uniform manifold approximation and projection (UMAP) representation of single-cell transcriptomes derived from E12.5 Smc3fl/fl, Smc3fl/+; Nkx2.5-Cre, and Smc3fl/fl; Nkx2.5-Cre embryonic cardiac tissue (b) and CMs (d). Dot plots showing the expression of individual marker genes across the cardiac tissue (c) and CM (e) lineages. The color from dark to light represents the average expression from high to low. The size of the dot represents the percentage of cells within that cluster expressing the indicated gene. f Heatmap illustrating scaled expression of CM differentiation master genes in CMs and OFT development master genes in outflow tract like (OFTL)-CMs from E12.5 Smc3fl/fl, Smc3fl/+; Nkx2.5-Cre, and Smc3fl/fl; Nkx2.5-Cre cardiac tissue. The color from dark to light indicates the scaled expression from low to high. g Bar plots displaying representative Gene Ontology (GO) terms in which downregulated genes were enriched in OFTL-CMs from E12.5 Smc3fl/+; Nkx2.5-Cre (top panel) and Smc3fl/fl; Nkx2.5-Cre (bottom panel) cardiac tissues. h Pseudotemporal ordering trajectory map of OFTL-CMs from Smc3fl/fl, Smc3fl/+; Nkx2.5-Cre, and Smc3fl/fl; Nkx2.5-Cre embryonic cardiac tissues. Dark to light colors represent the pseudotime order. Fb_like fibroblast-like cell, Epi epicardial cell, EC endothelial cell, A-CM atrium-cardiac muscle cell, LV-CM left ventricle-cardiac muscle cell, AVC-CM atrioventricular canal-cardiac muscle cell.
