Fig. 1: Stanniocalcin 1 (STC1) negatively regulates bone morphogenetic protein 2 (BMP2)-induced osteoblast differentiation and function.

a, b Osteoblast precursors transduced with pMX-FIG (control) or pMX-STC1 were cultured in osteogenic medium (OGM). a The cultured cells were stained with Alizarin Red (left) and quantified via extraction (right) (n = 3). b Relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). c, d Osteoblast precursors transfected with negative control siRNA (Con-si) or Stc1 siRNA (Stc1-si) were cultured in OGM. c The cultured cells were stained with Alizarin Red (left) and quantified via extraction (right) (n = 3). d Relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). The data are presented as the means ± SDs of triplicate samples. #p < 0.05, *p < 0.01, **p < 0.001 vs. the control.