Fig. 2: STC1 increases 1,25(OH)2D3 (Vit. D3)-mediated osteoclast formation.
a Osteoblast precursors transduced with pMX-FIG (control) or pMX-STC1 were cocultured with bone marrow cells in the presence or absence of Vit. D3 (10−8 M) or Vit. D3 + prostaglandin E2 (PGE2, 10−7 M). The cultured cells were stained with tartrate-resistant acid phosphatase (TRAP, left), and TRAP-positive MNCs were quantified (right) (n = 3). Bar, 200 µm. b Osteoblast precursors transduced with pMX-FIG (control) or pMX-STC1 were cultured in the presence or absence of Vit. D3 (10−8 M). The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). c Osteoblast precursors transfected with Con-si or Stc1-si were cocultured with bone marrow cells in the presence or absence of Vit. D3 (10−8 M) or Vit. D3 (10−8 M) + PGE2 (10−7 M). The cultured cells were stained with TRAP (left), and TRAP-positive MNCs were quantified (right) (n = 3). Bar, 200 µm. d Osteoblast precursors transfected with Con-si or Stc1-si were cultured in the presence or absence of Vit. D3 (10−8 M). The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). e, f Osteoblast precursors transduced with pMX-FIG (control) or pMX-STC1 were cultured in OGM in the presence or absence of Vit. D3 (10−8 M). e The cultured cells were stained with Alizarin Red (upper) and quantified via extraction (lower) (n = 3). f Relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). g, h Osteoblast precursors transfected with Con-si or Stc1-si were cultured in OGM in the presence or absence of Vit. D3 (10−8 M). g The cultured cells were stained with Alizarin Red (left) and quantified via extraction (right) (n = 3). h Relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). The data are presented as the means ± SDs of triplicate samples. #p < 0.05, *p < 0.01, **p < 0.001 vs. the control.
