Fig. 5: Vit. D3 positively regulates STC1 expression through transcriptional regulation. | Experimental & Molecular Medicine

Fig. 5: Vit. D3 positively regulates STC1 expression through transcriptional regulation.

From: Stanniocalcin 1 and 1,25-dihydroxyvitamin D3 cooperatively regulate bone mineralization by osteoblasts

Fig. 5

a Osteoblast precursors transfected with Con-si or Vdr-si were cultured in the presence or absence of Vit. D3 (10−8 M). The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). b Osteoblast precursors were transfected with Con-si or Vdr-si and then cultured in OGM + Vit. D3 (10−8 M). The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). c Immunoprecipitation was performed using anti-VDR antibodies or IgG as the negative control. Precipitated DNA was subjected to PCR with primers targeting the VDR binding site (−1057 to −1069). The data are presented as the means ± SDs of triplicate samples. #p < 0.05, *p < 0.01, **p < 0.001 vs. the control.

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