Fig. 6: STC1 and Vit. D3 inhibit ectopic bone formation in vivo.
a, b Collagen sponges soaked with BMP2 (1.2 µg) with or without STC1 (3 µg) were subcutaneously implanted on the top of the dorsal back. Ectopic bones were biopsied and subjected to µCT analyses. a Representative 3D images of ectopic bones analyzed by µCT. b Bone volume (BV), bone volume/tissue volume (BV/TV), and bone area were determined by µCT (n = 6). c, d Collagen sponges soaked with BMP2 (1.2 µg) with or without Vit. D3 (0.04 µg) were subcutaneously implanted on the top of the dorsal back. Ectopic bones were biopsied and subjected to µCT analyses.0 c Representative 3D images of ectopic bones analyzed by µCT. d BV, BV/TV, and bone area were determined by µCT (n = 12). e, f Collagen sponges soaked with BMP2 (1.2 µg) and Vit. D3 (0.04 µg) with or without STC1 (3 µg) were subcutaneously implanted on the top of the dorsal back. Ectopic bones were biopsied and subjected to µCT analyses. e Representative 3D images of ectopic bones analyzed by µCT. f BV, BV/TV, and bone area were determined by µCT (n = 6). The data are presented as the means ± SDs. #p < 0.05, *p < 0.01, **p < 0.001 vs. the control.
