Fig. 7: Stc1-overexpressing transgenic mice exhibit a mild decrease in long bone mass. | Experimental & Molecular Medicine

Fig. 7: Stc1-overexpressing transgenic mice exhibit a mild decrease in long bone mass.

From: Stanniocalcin 1 and 1,25-dihydroxyvitamin D3 cooperatively regulate bone mineralization by osteoblasts

Fig. 7

a Bone marrow stromal cells (BMSCs) isolated from STC1 transgenic (Tg) mice or their wild-type littermates were cultured in the presence or absence of OGM. The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). b–e Long bones of STC1 Tg mice or their wild-type littermates were subjected to µCT and histological analyses. (b) Representative 3D images of femurs from STC1 Tg mice or their wild-type littermates, as analyzed by µCT. c Bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), cortical bone volume/tissue volume (Corti BV/TV), and cortical thickness (Corti Th) were determined by µCT (n = 5 or 7). d H&E- and TRAP-stained images of tibiae isolated from STC1 Tg mice or their wild-type littermates. e Quantification of osteoblast number and osteoclast number derived from histological analyses (n = 5 or 7). The data are presented as the means ± SDs. #p < 0.05, *p < 0.01, **p < 0.001 vs. the control.

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