Fig. 8: Osteoblastic STC1 overexpression reduces bone mass in the presence of Vit. D3 administration in vivo.
a Osteoblasts were induced from BMSCs isolated from STC1 Tg mice or their wild-type littermates. The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). b BMSCs isolated from STC1 Tg mice or their wild-type littermates were cultured in the presence or absence of Vit. D3 (10−8 M). The relative mRNA expression levels of the indicated genes were determined by real-time PCR (n = 3). c–f Long bones of STC1 Tg mice or their wild-type littermates were intraperitoneally injected with Vit. D3 and subjected to µCT and histological analyses. c Representative 3D images of femurs from STC1 Tg mice or their wild-type littermates intraperitoneally injected with Vit. D3, as analyzed by µCT. d Bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), cortical bone volume/tissue volume (Corti BV/TV), and cortical thickness (Corti Th) were determined by µCT (n = 6 or 7). e H&E- and TRAP-stained images of tibiae isolated from STC1 Tg mice or their wild-type littermates intraperitoneally injected with Vit.D3. f Quantification of osteoblast number and osteoclast number derived from histological analyses (n = 6 or 7). The data are presented as the means ± SDs. #p < 0.05, *p < 0.01 vs. the control.
