Fig. 2: ScRNA-seq analysis of noncardiomyocytes from heart failure patients with hyperlipidemia and hypertension. | Experimental & Molecular Medicine

Fig. 2: ScRNA-seq analysis of noncardiomyocytes from heart failure patients with hyperlipidemia and hypertension.

From: Altered lipid metabolism promoting cardiac fibrosis is mediated by CD34+ cell-derived FABP4+ fibroblasts

Fig. 2

a Schematic depicting the pipeline of human heart and tissue harvesting for scRNA-seq. Single cells were then isolated from the left ventricle and subjected to scRNA-seq. b UMAP plot displaying the major cell types and color-coded cell clusters of human hearts. c Bar chart showing the percentages of major cell types among different datasets. d UMAP plot displaying the distribution of lipids according to GSVA. e UMAP plot displaying the distribution of fibroblast subpopulations. Resolution = 0.5. f Violin plot showing the expression of selected marker genes in each subcluster. g GO analysis of Cluster 5 showed that FABP4+ fibroblasts were enriched in the triglyceride metabolism pathway (R-HAS-163560, circled by the red rectangle). h, i Representative images showing specific cells identified by staining for POSTN, FABP4, and PDGFRa. Scale bars, 50 μm and 20 μm in magnified images. j mRNA levels of a fibroblastic marker (Postn) and FABP4 in total fibroblasts isolated from patient hearts. GAPDH was used as an internal control, n = 4. The data are given as mean ± SEM. P < 0.05 was considered to indicate statistical significance.

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