Fig. 3: scRNA-seq analysis of noncardiomyocytes from ApoE-/- mice with hyperlipidemia and hypertension. | Experimental & Molecular Medicine

Fig. 3: scRNA-seq analysis of noncardiomyocytes from ApoE-/- mice with hyperlipidemia and hypertension.

From: Altered lipid metabolism promoting cardiac fibrosis is mediated by CD34+ cell-derived FABP4+ fibroblasts

Fig. 3

a Wheat germ agglutinin (WGA) staining showing cardiomyocyte cell size. Scale bars, 50 μm and 20 μm in magnified images. b. UMAP plot displaying the major cell types and color-coded cell clusters of mouse hearts; UMAP plot displaying the distribution of lipids by GSVA. c UMAP plot displaying the distribution of fibroblast subpopulations among different datasets after Ang II surgery. Resolution = 0.5. d Heatmap showing the expression of the top ten differentially expressed genes in each cell subcluster. e Violin plot showing the expression of selected marker genes in each subcluster. f Violin plot showing the GO dataset scores among fibroblast subclusters. g Bar plot showing the GO enrichment of selected subclusters. h GSVA of fibroblasts from the three groups. i. Representative images showing specific cell identification by staining for Vimentin and Fabp4. Scale bars, 20 μm. j mRNA levels of Fabp4 in total fibroblasts isolated from mouse heart; GAPDH was used as an internal control. Data are given as the mean ± SEM, n = 5. P < 0.05 was considered to indicate statistical significance.

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