Fig. 5: scRNA-seq analysis revealed the role of lipid metabolism disturbance in myocardial fibrosis. | Experimental & Molecular Medicine

Fig. 5: scRNA-seq analysis revealed the role of lipid metabolism disturbance in myocardial fibrosis.

From: Altered lipid metabolism promoting cardiac fibrosis is mediated by CD34+ cell-derived FABP4+ fibroblasts

Fig. 5

a UMAP plot displaying the distribution of fibroblast subpopulations among different datasets. b Heatmap showing the expression of the top ten differentially expressed genes in each cell subcluster. c Violin plot showing the expression of selected marker genes in each subcluster. d Representative images showing specific cell identification by staining for Postn and Fabp4. Scale bars = 20 μm. e Violin plot showing the expression of FABP4 in fibroblasts in the two groups. f mRNA levels of fibroblastic markers (Postn, Vimentin, DDR2 and Fabp4) in the hearts of mice. GAPDH was used as an internal control. Data are presented as the mean ± SEM, n = 4. P < 0.05 was considered to indicate statistical significance. g Heatmap showing the expression of the top ten differentially expressed genes in each cell subcluster from the tdTomato+ cell dataset of these two groups. h, i Violin plot showing the expression of selected marker genes in each subcluster. j Representative images showing specific cell identification by staining for tdTomato and Fabp4. Scale bars, 50 μm. k Representative images showing specific cell identification by staining for Fabp4 and tdTomato. Scale bars = 50 μm.

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