Fig. 2: Total iron levels and intracellular iron localization in the liver tissue after MCAO. | Experimental & Molecular Medicine

Fig. 2: Total iron levels and intracellular iron localization in the liver tissue after MCAO.

From: Hepatocyte activation and liver injury following cerebral ischemia promote HMGB1-mediated hepcidin upregulation in hepatocytes and regulation of systemic iron levels

Fig. 2

a Liver tissue samples were obtained 3, 6, 12, 24, 48, and 72 h after MCAO. The total iron content was measured using an iron assay kit. The results are presented as the means ± SEMs (n = 4). Liver tissue sections were prepared from the sham control (b, c) and MCAO groups (d–g) at 12 and 24 h after MCAO and stained with Perls’ Prussian blue and nuclear fast red (a nuclear counterstain). Prussian blue-positive cells are indicated by arrows. Representative images from three MCAO groups are shown. The scale bars in b, d, and f represent 50 µm, and those in c, e, and g represent 20 µm. *p < 0.05 and **p < 0.01 compared with the sham-operated control group.

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